Previously, the terms sterile technique, clean technique and aseptic technique have been used interchangeably. To demonstrate skill in isolation of organisms from a mixed culture using selective and differential media. These terms, which can be obviously used in a medical setting, also apply to the safekeeping of. Semesterlong assessment of aseptic technique in microbiology. Biol 202each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit if the question asks you to do research or find a source, a reputable, credible andor scholarly source citation must be. Aseptic sterile technique used in microbiology laboratory.
This procedure is used for isolating bacterial colonies from mixed cell cultures and is by far one of the most important techniques to master in microbiology and molecular genetics. The difference between aseptic and sterile is not always properly understood. Use aseptic technique to inoculate sterile nutrient broth then incubate according to specifications of the bacterial strain. Remove the lid from the first agar plate and place the exposed agar portion in or out of the building for the duration of todays lab. Pdf biology educators emphasize aseptic technique when teaching lab courses. Refer to your laboratory text for various methods of transferring microbial cultures.
Although it can be assessed throughout the semester by examining the students technique and observation of contamination, this tool offers a simple semesterlong graded assessment of each students technique. Concepts in sterile preparations and aseptic technique. Although it can be assessed throughout the semester by examining the students technique and. Biol 202each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit if the question asks you to do research or find a source, a reputable, credible andor scholarly source. Introduction aseptic technique is used for several procedures by microbiologists. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Dec 02, 20 aseptic technique is very important in microbiology to ensure safety and prevent crosscontamination. Aseptic an environment or procedure that is free of contamination by pathogens bauman, robert w. Here are some examples of how this technique is practiced.
A sterile technique experiment was conducted to test the effects hand cleansing procedures has on contamination of agar plates. There may be images in this book that feature models. An introduction to microbiology, aseptic technique and safety as well as causing a familiar range of diseases in animals and plants and problems in food spoilage and deterioration of other materials, microbes are also our invisible allies. Because bacteria are relatively simple organisms and are amenable to experimental manipulation, they have provided elegant systems for basic research. Aseptic technique microbiology resource center truckee. State and define the three types of growth that may be seen in a broth culture. Aseptic technique is essentially the backbone of microbiology. The aseptic technique decreases infection risks for the patient directly at the source. Training topics should include hygiene, safety hazards, specific written procedures, regular ongoing programs, aseptic filling technique, cleanroom behavior, microbiology etc. However, it is extremely important to always practice aseptic technique to avoid culturing. Perform soft agar overlays when working with phage.
Aseptic technique bykowski 2008 current protocols in. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Basic practical microbiology why microbiology matters. Objectives to demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials. The handbook and videos provide an introduction to cell culture, with a focus on aseptic technique, microbial contamination, laboratory safety and cell culture experiments. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. They also include all of the prophylactic methods, working.
Bacteria can be isolated from nearly any surface including inanimate objects and human skin. Prevent contamination of the room and personnel with the microorganism we are working with. Jun 21, 2019 mixed cultures more than one species can be isolated using the streak plate technique. Choose from 500 different sets of aseptic technique microbiology flashcards on quizlet.
Microbiologists use aseptic technique for a variety of procedures such. Aseptic technique is absolutely necessary for the successful establishment and maintenance of plant cell, tissue and organ cultures. Learn aseptic technique microbiology with free interactive flashcards. An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2 good microbiological laboratory practice gmlp 3 spillage management 3 aerosols 3 resources equipment 4 apparatus 5 materials 5 media, sterilisation and disinfection preparation of culture media 6 pouring a plate 6 storage of media 6. Leftover cultures should be discarded at the end of the day. Aseptic technique is a set of best practices that healthcare professionals use to prevent the transfer of germs in clinics and hospitals. The goal is to reach asepsis, which means an environment that is free of harmful microorganisms. The gas burner consists of a vertical metal tube through which a narrow jet of natural gas is directed. Aseptic technique good aseptic or sterile technique is of critical importance in many areas of biology, particularly in microbiology. Lastly, it would be worth to note that some major techniques of maintaining sterility of all items and surfaces which are critical areas may include.
Introduction to microbiology, sterile technique and. Aseptic technique and the transfer of microorganisms theory. May 11, 2012 use aseptic technique to inoculate sterile nutrient broth then incubate according to specifications of the bacterial strain. Learn microbiology aseptic technique with free interactive flashcards. Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms i. Hygiene on patients skin is performed using alcohol or other antimicrobial cleanser when following aseptic technique. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. The in vitro environment in which the plant material is grown is also ideal for the proliferation of microorganisms. Choose from 500 different sets of microbiology aseptic technique flashcards on quizlet. Note that a microincinerator does not provide other aseptic technique benefits of a lit bunsen burner. Jan 14, 2020 general aseptic techniques in microbiology laboratory.
Aseptic technique is used for many procedures such as transferring cultures, inoculating media, and for performing a. While not necessarily sterile, aseptic technique goes beyond simple cleaning and clean technique. These procedures include transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. An introduction to microbiology, aseptic technique and safety. Clean bench area with swab soaked in methylated spirit. Bacteria represent the most widely distributed and metabolically diverse group of organisms.
The hypothesis for this experiment was that cultures inoculated with s. Aseptic technique is a procedure used by medical staff to prevent the spread of infection. A sample application for streak plating is shown in figure 1. To prevent the access of microorganisms during the preparation and testing. In order to illustrate that microorganisms are all around us and to demonstrate the necessity for proper aseptic technique, contaminate three trypticase soy agar plates as follows. Pdf semesterlong assessment of aseptic technique in. Aseptic technique is a set of principles and practices used by cell culture workers to reduce the presence of unwanted microorganisms or other cell lines in their cultures. Aseptic technique an overview sciencedirect topics. You have free access to a large collection of materials used in a collegelevel introductory microbiology course. Each colony represents a population of cells that are genetically identical. Practicing aseptic technique with microbial cultures flinn scientific. Dont touch any part of the container, pipette, etc.
Aseptic technique aseptic technique is a set of specific practices and procedures performed under carefully controlled conditions with the goal of minimizing contamination by pathogens. Jun 12, 2017 aseptic technique is essentially the backbone of microbiology. Indeed, life on earth would not be sustainable without the. Aseptic refers to the absence of contamination with germs. Christie t ammirati md, in surgery of the skin, 2005. Nov 08, 2018 aseptic technique is a set of best practices that healthcare professionals use to prevent the transfer of germs in clinics and hospitals and protect patients from infection. As we have seen in the previous exercise, contamination is a serious concern in the microbiology laboratory. The aseptic techniques control the opportunities for contamination of cultures by microorganisms from the environment, or contamination of. All bacterial cultures sold by flinn scientific are nonpathogenic. Culturing and aseptic technique 100 points student name. Good aseptic technique is essential for successful longterm cell and tissue culture.
Prevent contamination of the specific microorganism we are working with. Aseptic technique steps, purpose and importance of aseptic. General aseptic techniques in microbiology laboratory basic. Media and equipment selection sterile work area for labs with cell culture hoods. Aseptic technique and the transfer of microorganisms, virtual amrita laboratories microbiology lab flashcards from quizlet when clinical samples are plated, a sterile swab is used to obtain the biological sample from the patient such as throat swab and to transfer that sample to a plate. Aseptic technique and the transfer of microorganisms. In the microbiology lab we use aseptic technique to. Aseptic techniques refer to any method used to sterilize and maintain. This chapter describes common laboratory procedures that can reduce the risk of culture con taminations sepsis, collectively referred as aseptic technique. Concepts in sterile preparations and aseptic technique is an independent publication and has not been authorized, sponsored, or otherwise approved by the owners of the trademarks or service marks referenced in this product. Proper aseptic technique prevents contamination of cultures from. Preventing contamination of experimental materials with microbes from the environment, including from your body. It is a fundamental skill for working in a microbiology laboratory. Two major strategies of aseptic work are described.
The operators aseptic technique may deteriorate with the passage of time. General aseptic techniques in microbiology laboratory. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. Aseptic and sterile conditions are different from each other because one should free form pathogens and other should free from microbes.
Asepsis covers all those procedures designed to reduce the risk of bacterial, fungal or viral contamination, using sterile instruments, sterile draping and the gloved no touch technique. Feb 26, 2019 aseptic techniques refer to any method used to sterilize and maintain the sterility of an object or location. Sterile work area for labs with cell culture hoods. An introduction to microbiology, aseptic technique and safety as well as causing a familiar range of diseases in animals and plants and problems in food spoilage and deterioration. Aseptic technique is a means of performing lab work that greatly reduces the risk of contamination. The simplest and most economical way to reduce contamination from airborne particles and aerosols e. Aseptic technique is the range of infection prevention and control practices which are used to minimize the presence of pathogenic microorganisms during clinical procedures. Microorganisms in the lab atmosphere may come to rest on the desktop between. What is the role of aseptic techniques in a microbiology lab. Introduction to microbiology, sterile technique and micropipetting background. Transfer bacterial cells from one plate to another using the replicaplating procedure. Aseptic technique, designed to provide a barrier between the microorganisms in the environment and the sterile cell culture, depends upon a set of procedures to reduce the probability of contamination from these sources.
Aseptic technique involves developing both manual dexterity in safely handling the microorganisms and mental dexterity in thinking ahead about what you are. Mixed cultures more than one species can be isolated using the streak plate technique. Many of the organisms can be opportunistic in their abilities to cause infection. This technique ensures a contaminant free environment while handling micro organisms. Aseptic techniques aseptic techniques are the precautionary measures taken to prevent contamination of pure cultures and sterile laboratory equipment. Thus, this experiment is a very important one which serves as basics for other microbiology procedures. Prevent contamination of the room and personnel with the. Preventing surgical site infection in the operating room is the primary goal of the surgical team, and all activities performed by the team support this goal. Module context this module introduces microbiological laboratory techniques to be used for analysis of coliforms bacteria can be used as indicators of pollution. Aseptic technique is very important in microbiology to ensure safety and.
There is another technique called as clean technique which is applied to prevent the further spread of microorganisms between other people and places. Many of the microorganisms we will be working with in lab are known pathogens. Aseptic technique is very important in microbiology to ensure safety and prevent crosscontamination. Air is drawn in via air holes located near the stand. Asepsis is a condition in which no living diseasecausing microorganisms are present. Issue 63, microbiology, aseptic technique, sterile field, serological pipette.